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ProductsBack/HSPB6 Recombinant Antibody, Biotin Conjugated

HSPB6 Recombinant Antibody, Biotin Conjugated

BSM-61344R-BIOTIN
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSPB6
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HSPB6 Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID126393
  • Target name
    HSPB6
  • Target description
    heat shock protein family B (small) member 6
  • Target synonyms
    epididymis luminal protein 55; epididymis secretory sperm binding protein; heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6; HEL55; Hsp20; PPP1R91; protein phosphatase 1, regulatory subunit 91
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14558
  • Protein Name
    Heat shock protein beta-6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hsp20 using anti-Hsp20 antibody (A07981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysate,Lane 2: rat skeletal muscle tissue lysate,Lane 3: rat testis tissue lysate,Lane 4: mouse testis tissue lysate,Lane 5: human A431 whole Cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp20 antigen affinity purified polyclonal antibody (Catalog # A07981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp20 at approximately 17KD. The expected band size for Hsp20 is at 17KD.
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