![IHC-P analysis of human breast carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500 IHC-P analysis of human breast carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_1113_IHC-P_w_23060620_380.webp)
IHC-P analysis of human breast carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500
HSP27 antibody [G3.1]
GTX22790
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
TargetHSPB1
Overview
- SupplierGeneTex
- Product NameHSP27 antibody [G3.1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:1000. ICC/IF: 1:50 - 1:500. IHC-P: 1:500. IP: 2 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDG3.1
- ConjugateUnconjugated
- Gene ID3315
- Target nameHSPB1
- Target descriptionheat shock protein family B (small) member 1
- Target synonymsCMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27, heat shock protein beta-1, 28 kDa heat shock protein, epididymis secretory protein Li 102, estrogen-regulated 24 kDa protein, heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein family B member 1, stress-responsive protein 27
- HostMouse
- IsotypeIgG1
- Protein IDP04792
- Protein NameHeat shock protein beta-1
- Scientific DescriptionThis gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human colon carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of human colon carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_1114_IHC-P_w_23060620_186.webp)
![IHC-P analysis of human tonsil tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500 IHC-P analysis of human tonsil tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_1115_IHC-P_w_23060620_862.webp)
![IP analysis of HeLa cell lysates using GTX22790 HSP27 antibody [G3.1]. GTX22790 was used as IP and IB antibody IP reaction : 2microg antibody / 500microg lysate WB antibody dilution : 1:1000 IP analysis of HeLa cell lysates using GTX22790 HSP27 antibody [G3.1]. GTX22790 was used as IP and IB antibody IP reaction : 2microg antibody / 500microg lysate WB antibody dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_1433_IP_w_23060620_847.webp)
![WB analysis of 50microg of the indicated whole cell lysates using GTX22790 HSP27 antibody [G3.1]. Dilution : 1:1000 WB analysis of 50microg of the indicated whole cell lysates using GTX22790 HSP27 antibody [G3.1]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_1594_WB_w_23060620_922.webp)
![ICC/IF analysis of HeLa cells and negative control NIH3T3 cells using GTX22790 HSP27 antibody [G3.1]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature ICC/IF analysis of HeLa cells and negative control NIH3T3 cells using GTX22790 HSP27 antibody [G3.1]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_446_ICC-IF_w_23060620_410.webp)
![ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_447_ICC-IF_w_23060620_477.webp)
![ICC/IF analysis of C6 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC ICC/IF analysis of C6 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_448_ICC-IF_w_23060620_469.webp)
![ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_449_ICC-IF_w_23060620_998.webp)
![ICC/IF analysis of U251 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC ICC/IF analysis of U251 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22790/GTX22790_450_ICC-IF_w_23060620_628.webp)








![ELISA analysis of antigen using GTX60444 HSP27 antibody [5D7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_ELISA_w_23061123_806.webp)