![FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34785 HSP60 antibody [LK1]. Blue : Primary antibody Red : Isotype control FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34785 HSP60 antibody [LK1]. Blue : Primary antibody Red : Isotype control](https://www.genetex.com/upload/website/prouct_img/normal/GTX34785/GTX34785_20200115_FACS_1653_w_23060801_985.webp)
FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34785 HSP60 antibody [LK1]. Blue : Primary antibody Red : Isotype control
HSP60 antibody [LK1]
GTX34785
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSPD1
Overview
- SupplierGeneTex
- Product NameHSP60 antibody [LK1]
- Delivery Days Customer9
- Application Supplier NoteWB: 0.25-0.5microg/ml. ICC/IF: 1-2microg/ml. IHC-P: 1-2microg/ml for 30 minutes at RT. FACS: 1-2microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDLK1
- Concentration0.2 mg/ml
- ConjugateUnconjugated
- Gene ID3329
- Target nameHSPD1
- Target descriptionheat shock protein family D (Hsp60) member 1
- Target synonyms60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); heat shock protein 65; HLD4; HSP60; HSP-60; HSP65; HuCHA60; mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13
- HostMouse
- IsotypeIgG1
- Protein IDP10809
- Protein Name60 kDa heat shock protein, mitochondrial
- Scientific DescriptionThis gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
- Storage Instruction2°C to 8°C
- UNSPSC12352203
![ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34785 HSP60 antibody [LK1]. Green : Primary antibody Red : nucleus ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34785 HSP60 antibody [LK1]. Green : Primary antibody Red : nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX34785/GTX34785_20200115_ICC-IF_1733_w_23060801_136.webp)
![IHC-P analysis of human breast carcinoma tissue using GTX34785 HSP60 antibody [LK1]. IHC-P analysis of human breast carcinoma tissue using GTX34785 HSP60 antibody [LK1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34785/GTX34785_20200115_IHC-P_368_w_23060801_479.webp)
![IHC-P analysis of human pancreas tissue using GTX34785 HSP60 antibody [LK1]. IHC-P analysis of human pancreas tissue using GTX34785 HSP60 antibody [LK1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34785/GTX34785_20200115_IHC-P_879_w_23060801_211.webp)
![WB analysis of HeLa cell lysate using GTX34785 HSP60 antibody [LK1]. WB analysis of HeLa cell lysate using GTX34785 HSP60 antibody [LK1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34785/GTX34785_20200115_WB_121_w_23060801_764.webp)

![ELISA analysis of antigen using GTX60424 HSP60 antibody [3G8]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_ELISA_w_23061123_845.webp)
![ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34786 HSP60 antibody [SPM253]. Green : Primary antibody Red : nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX34786/GTX34786_20200115_ICC-IF_1743_w_23060801_701.webp)
![ICC/IF analysis of MeOH-fixed HeLa cells using GTX34788 HSP60 antibody [HSPD1/780]. Green : Primary antibody Red : nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX34788/GTX34788_20200115_ICC-IF_1586_w_23060801_947.webp)
![IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1292_IHC-P_w_23060722_302.webp)
![IHC-P analysis of human breast carcinoma tissue using GTX25479 HSP60 antibody [2E1/53]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25479/GTX25479_1295_IHC-P_w_23060722_125.webp)

