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HSP75 Polyclonal Antibody

E-AB-65477
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HSP75 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAP1 using anti-TRAP1 antibody (A02426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP1 antigen affinity purified polyclonal antibody (Catalog # A02426-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAP1 at approximately 80 kDa. The expected band size for TRAP1 is at 80 kDa.
Product group Antibodies
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References
GeneTex
TRAP1 antibodyGTX102017
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ReactivityHuman, Mouse
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Immunohistochemical staining of human cerebellum, fallopian tube, kidney and testis using Anti-TRAP1 antibody HPA041082 (A) shows similar protein distribution across tissues to independent antibody HPA044227 (B).
Product group Antibodies
Atlas Antibodies
Anti-TRAP1 AntibodyHPA041082
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTRAP1
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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Mouse heart tissue All lanes: TRAP1 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 75 kDa Observed band size: 81 kDa
Product group Antibodies
Cusabio
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ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
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Product group Antibodies
MedChem Express
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