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HSP90B1 / GP96 / GRP94 Antibody (Preservative Free)

LS-C815137
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHSP90B1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HSP90B1 / GP96 / GRP94 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7184
  • Target name
    HSP90B1
  • Target description
    heat shock protein 90 beta family member 1
  • Target synonyms
    94 kDa glucose-regulated protein; ECGP; endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; GP96; GRP94; heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; HEL35; HEL-S-125m; stress-inducible tumor rejection antigen gp96; TRA1; tumor rejection antigen (gp96) 1; tumor rejection antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRP94 using anti-GRP94 antibody (PB9637). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: huamn CACO-2 whole cell lysates, Lane 5: huamn THP-1 whole cell lysates, Lane 6: huamn CACO-2 whole cell lysates, Lane 7: huamn HepG2 whole cell lysates, Lane 8: huamn HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94 antigen affinity purified polyclonal antibody (Catalog # PB9637) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94 at approximately 100 kDa. The expected band size for GRP94 is at 92 kDa.
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