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Western Blot analysis of Hela cell and Human liver cancer tissue, Jurkat and NIH/3T3 cell using HSP90B1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela cell and Human liver cancer tissue, Jurkat and NIH/3T3 cell using HSP90B1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela cell and Human liver cancer tissue, Jurkat and NIH/3T3 cell using HSP90B1 Polyclonal Antibody at dilution of 1:500

HSP90B1 Polyclonal Antibody

E-AB-10353
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHSP90B1
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Overview

  • Supplier
    Elabscience
  • Product Name
    HSP90B1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7184
  • Target name
    HSP90B1
  • Target description
    heat shock protein 90 beta family member 1
  • Target synonyms
    ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1, endoplasmin, 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, epididymis luminal protein 35, epididymis secretory sperm binding protein Li 125m, heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, heat shock protein 90kDa beta family member 1, heat shock protein family C member 4, stress-inducible tumor rejection antigen gp96, tumor rejection antigen (gp96) 1, tumor rejection antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14625
  • Protein Name
    Endoplasmin
  • Scientific Description
    This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5 exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of GRP94 using anti-GRP94 antibody (PB9637). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: huamn CACO-2 whole cell lysates, Lane 5: huamn THP-1 whole cell lysates, Lane 6: huamn CACO-2 whole cell lysates, Lane 7: huamn HepG2 whole cell lysates, Lane 8: huamn HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94 antigen affinity purified polyclonal antibody (Catalog # PB9637) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94 at approximately 100 kDa. The expected band size for GRP94 is at 92 kDa.
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