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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate All lanes: HSPA12A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate All lanes: HSPA12A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate All lanes: HSPA12A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa

HSPA12A Antibody

CSB-PA010817LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetHSPA12A
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPA12A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID259217
  • Target name
    HSPA12A
  • Target description
    heat shock protein family A (Hsp70) member 12A
  • Target synonyms
    heat shock 70 kDa protein 12A; heat shock 70kD protein 12A; heat shock 70kDa protein 12A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43301
  • Protein Name
    Heat shock 70 kDa protein 12A
  • Scientific Description
    extracellular exosome
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSPA12A using anti-HSPA12A antibody (A13632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA12A antigen affinity purified polyclonal antibody (Catalog # A13632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA12A at approximately 75 kDa. The expected band size for HSPA12A is at 75 kDa.
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