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ProductsBack/HSPA4 Mouse Monoclonal Antibody [Clone ID: 5A6]
Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.
Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.
Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.

HSPA4 Mouse Monoclonal Antibody [Clone ID: 5A6]

AM06455SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSPA4
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    HSPA4 Mouse Monoclonal Antibody [Clone ID: 5A6]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A6
  • Gene ID3308
  • Target name
    HSPA4
  • Target description
    heat shock protein family A (Hsp70) member 4
  • Target synonyms
    APG-2, HEL-S-5a, HS24/P52, HSPH2, RY, hsp70, hsp70RY, heat shock 70 kDa protein 4, epididymis secretory sperm binding protein Li 5a, heat shock 70-related protein APG-2, heat shock 70kD protein 4, heat shock protein family H member 2, heat shock protein, 110 kDa, hsp70 RY
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP34932
  • Protein Name
    Heat shock 70 kDa protein 4
  • Scientific Description
    HSPA4 mouse monoclonal antibody, clone 5A6, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HSPA4 using anti-HSPA4 antibody (A03618-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA4 antigen affinity purified polyclonal antibody (Catalog # A03618-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for HSPA4 at approximately 110 kDa. The expected band size for HSPA4 is at 96,14 kDa.
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