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Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue All lanes: HSPB6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue All lanes: HSPB6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue All lanes: HSPB6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa

HSPB6 Antibody

CSB-PA010837LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetHSPB6
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPB6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID126393
  • Target name
    HSPB6
  • Target description
    heat shock protein family B (small) member 6
  • Target synonyms
    HEL55, Hsp20, PPP1R91, heat shock protein beta-6, epididymis luminal protein 55, epididymis secretory sperm binding protein, heat shock 20 kDa-like protein p20, heat shock protein family B (small) member B6, heat shock protein family B member 6, heat shock protein, alpha-crystallin-related, B6, protein phosphatase 1, regulatory subunit 91
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14558
  • Protein Name
    Heat shock protein beta-6
  • Scientific Description
    cytoplasm, nucleus, protein homodimerization activity
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hsp20 using anti-Hsp20 antibody (A07981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysate,Lane 2: rat skeletal muscle tissue lysate,Lane 3: rat testis tissue lysate,Lane 4: mouse testis tissue lysate,Lane 5: human A431 whole Cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp20 antigen affinity purified polyclonal antibody (Catalog # A07981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp20 at approximately 17KD. The expected band size for Hsp20 is at 17KD.
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