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Western Blot analysis of 3T3 cells using HSP10 Polyclonal Antibody
Western Blot analysis of 3T3 cells using HSP10 Polyclonal Antibody
Western Blot analysis of 3T3 cells using HSP10 Polyclonal Antibody

HSPE1 Antibody

CSB-PA009331
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3336
  • Target name
    HSPE1
  • Target description
    heat shock protein family E (Hsp10) member 1
  • Target synonyms
    CPN10, EPF, GROES, HSP10, 10 kDa heat shock protein, mitochondrial, 10 kDa chaperonin, chaperonin 10, early-pregnancy factor, epididymis secretory sperm binding protein, heat shock 10kD protein 1 (chaperonin 10), heat shock 10kDa protein 1, heat shock protein family E member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61604
  • Protein Name
    10 kDa heat shock protein, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSP10 using anti-HSP10 antibody (M06192-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP10 antigen affinity purified monoclonal antibody (Catalog # M06192-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP10 at approximately 11, 13 kDa. The expected band size for HSP10 is at 11 kDa.
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