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IHC-P analysis of human liver tissue using GTX85675 HTRA3 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 10 microg/ml
IHC-P analysis of human liver tissue using GTX85675 HTRA3 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 10 microg/ml
IHC-P analysis of human liver tissue using GTX85675 HTRA3 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 10 microg/ml

HTRA3 antibody

GTX85675
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHTRA3
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Overview

  • Supplier
    GeneTex
  • Product Name
    HTRA3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 3 microg/ml. IHC-P: 10 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID94031
  • Target name
    HTRA3
  • Target description
    HtrA serine peptidase 3
  • Target synonyms
    Prsp, Tasp, serine protease HTRA3, high-temperature requirement factor A3, pregnancy-related serine protease, probable serine protease HTRA3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP83110
  • Protein Name
    Serine protease HTRA3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HtrA3 using anti-HtrA3 antibody (A05478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PANC-1 whole Cell lysates, Lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HtrA3 antigen affinity purified polyclonal antibody (Catalog # A05478) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HtrA3 at approximately 49KD. The expected band size for HtrA3 is at 49KD.
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