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ProductsBack/Purified Anti-Human CD21 Antibody [BU32]

Purified Anti-Human CD21 Antibody [BU32]

AGEL0201
Assay Genie
ApplicationsFlow Cytometry, ImmunoHistoChemistry
Product group Antibodies
TargetCR2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Human CD21 Monoclonal Antibody [BU32] (AGEL0201)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoHistoChemistry
  • Applications Supplier
    FCM,IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1380
  • Target name
    CR2
  • Target description
    complement C3d receptor 2
  • Target synonyms
    C3DR; CD21; complement component (3d/Epstein Barr virus) receptor 2; complement component 3d receptor 2; complement receptor type 2; CR; CVID7; EBV receptor; SLEB9
  • Host
    Mouse
  • Protein IDP20023
  • Protein Name
    Complement receptor type 2
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity Supplier
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD21/CR2 using anti-CD21/CR2 antibody (A01632-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Raw264.7 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD21/CR2 antigen affinity purified polyclonal antibody (Catalog # A01632-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD21/CR2 at approximately 100,150 kDa. The expected band size for CD21/CR2 is at 113 kDa.
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