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ProductsBack/Human Laminin ELISA Kit EZ-Set(tm) (DIY Antibody Pairs)
Human Laminin EZ-Set ELISA Kit standard curve
Human Laminin EZ-Set ELISA Kit standard curve
Human Laminin EZ-Set ELISA Kit standard curve

Human Laminin ELISA Kit EZ-Set(tm) (DIY Antibody Pairs)

EZ0434
Boster Bio
ApplicationsELISA
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLAMA2
5 plates
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Human LAMA1 ez-set TM , ELISA Kit (DIY Antibody Pairs)
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Assay Detection Range
    156 pg/ml - 10,000 pg/ml
  • Certification
    Research Use Only
  • Gene ID3908
  • Target name
    LAMA2
  • Target description
    laminin subunit alpha 2
  • Target synonyms
    laminin M chain; laminin subunit alpha-2; laminin, alpha 2; laminin-12 subunit alpha; laminin-2 subunit alpha; laminin-4 subunit alpha; LAMM; MDC1A; merosin heavy chain; mutant laminin subunit alpha 2
  • Protein IDA4D0S4
  • Protein Name
    Laminin subunit beta-4
  • Scientific Description
    Human Laminin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human Laminin in cell culture supernatants, cell lysates and serum.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Laminin using anti-Laminin antibody (A03522-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody (Catalog # A03522-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin at approximately 200 kDa. The expected band size for Laminin is at 177-200 kDa.
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