The chemotactic effect of GTX00415-pro Human MCP1 / CCL2 protein (active). Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 25 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (C) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 50 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. Lower: The number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter).
The chemotactic effect of GTX00415-pro Human MCP1 / CCL2 protein (active). Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 25 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (C) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 50 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. Lower: The number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter).
Human MCP1 / CCL2 protein (active)
GTX00415-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Protein IDP13500
Overview
- SupplierGeneTex
- Product NameHuman MCP1 / CCL2 protein (active)
- Delivery Days Customer9
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Protein IDP13500
- Protein NameC-C motif chemokine 2
- Scientific DescriptionThis gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352202