Human MIP1 alpha protein, His tag (active)

GeneTex

Human MIP1 alpha protein (active)

9

MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 microl cell suspension, 1x106 cells/ml in RPMI-1640 with 0. 5% FBS) and MIP-1a (100 ng/ml, diluted in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8microm pore size) used to separate the two compartments. After incubation at 37C with 5% CO2 for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). By counting migrated cells in low chamber at high magnification (x400) randomly. A mean of 41. 2 THP-1 cells/field migrated towards serum free RPMI-1640 medium with 100 ng/ml MIP-1a, while only 3. 6 THP-1 cells/field migrated towards serum free RPMI-1640 medium.

Functional Assay

Research Use Only

Unconjugated

C-C motif chemokine 3

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

-20°C or -80°C,2°C to 8°C

12352202