Human sRANkL(Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand) ELISA Kit

Biorbyt

Human sRANkL(Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand) ELISA Kit

10

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sRANkL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sRANkL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sRANkL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human sRANkL in the samples is then determined by comparing the OD of the samples to the standard curve

15.63-1000 pg/mL

6.3 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand(sRANkL). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand(sRANkL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand(sRANkL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand(sRANkL) in the samples is then determined by comparing the OD of the samples to the standard curve.

Human

41116158