WB analysis of endogenous HTT lysates with or without different caspase activity (Lanes 1-5) and overexpressed recombinant HTT fragment lysates (Lanes 6-9) using a N-terminal pan-HTT antibody (upper panel) or GTX54521 Huntingtin (neoepitope 552) antibody at 1:500 (lower panel). Loading : 20 microg
WB analysis of endogenous HTT lysates with or without different caspase activity (Lanes 1-5) and overexpressed recombinant HTT fragment lysates (Lanes 6-9) using a N-terminal pan-HTT antibody (upper panel) or GTX54521 Huntingtin (neoepitope 552) antibody at 1:500 (lower panel). Loading : 20 microg
Huntingtin (neoepitope 552) antibody
GTX54521
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse
TargetHTT
Overview
- SupplierGeneTex
- Product NameHuntingtin (neoepitope 552) antibody
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes the 552 cleaved fragment without detecting the full-length form.
- Application Supplier NoteWB: 1:500. ICC/IF: 1:50-1:200. IHC-Fr: 1:50 : 1:200. ELISA: 1:20 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.4 mg/ml
- ConjugateUnconjugated
- Gene ID3064
- Target nameHTT
- Target descriptionhuntingtin
- Target synonymsHD; huntingtin; huntington disease protein; IT15; LOMARS
- HostRabbit
- IsotypeIgG
- Protein IDP42858
- Protein NameHuntingtin
- Scientific DescriptionHuntingtin is a disease gene linked to Huntingtons disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntingtons disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5 UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2016]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203