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WB analysis of HepG2 cells using GTX45354 IARS antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX45354 IARS antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX45354 IARS antibody at 0.2-1microg/ml.

IARS antibody, Internal

GTX45354
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetIARS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    IARS antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3376
  • Target name
    IARS1
  • Target description
    isoleucyl-tRNA synthetase 1
  • Target synonyms
    GRIDHH, IARS, ILERS, ILRS, IRS, PRO0785, isoleucine--tRNA ligase, cytoplasmic, isoleucine tRNA ligase 1, cytoplasmic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41252
  • Protein Name
    Isoleucine--tRNA ligase, cytoplasmic
  • Scientific Description
    Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IARS1 using anti-IARS1 antibody (A06805-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IARS1 antigen affinity purified polyclonal antibody (Catalog # A06805-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IARS1 at approximately 144 kDa. The expected band size for IARS1 is at 144 kDa.
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