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ICAM-1/CD54 Antibody

CAB5597
Assay Genie
TargetICAM1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    ICAM-1/CD54 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3383
  • Target name
    ICAM1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    BB2; CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor; P3.58
  • Host
    Rabbit
  • Protein IDP05362
  • Protein Name
    Intercellular adhesion molecule 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.
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