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Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

ICAM1 Polyclonal Antibody

BS-0608R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIcam1
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Overview

  • Supplier
    Bioss
  • Product Name
    ICAM1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID15894
  • Target name
    Icam1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    CD54, Icam-1, Ly-47, MALA-2, intercellular adhesion molecule 1, myD10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13597
  • Protein Name
    Intercellular adhesion molecule 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Shim W, Kim CE, Lee M, et al. Catechin solubilization by spontaneous hydrogen bonding with poly(ethylene glycol) for dry eye therapeutics. J Control Release. 2019,307:413-422. doi: 10.1016/j.jconrel.2019.04.016
    Read this paper
  • Li X, Liu Y, Guan W, et al. Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge. in loperamide-induced rats. J Ethnopharmacol. 2019,240:111961. doi: 10.1016/j.jep.2019.111961
    Read this paper
  • Zhang P, Hua L, Hou H, et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. Int J Mol Med. 2018,42(6):3344-3354. doi: 10.3892/ijmm.2018.3888
    Read this paper
  • Kim SC, Ko YS, Lee HY, et al. Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury. Korean J Intern Med. 2017,32(6):1053-1061. doi: 10.3904/kjim.2016.060
    Read this paper
  • Osada-Oka M, Shiota M, Izumi Y, et al. Macrophage-derived exosomes induce inflammatory factors in endothelial cells under hypertensive conditions. Hypertens Res. 2017,40(4):353-360. doi: 10.1038/hr.2016.163
    Read this paper
  • Ji M, Wang Y, Wang L, et al. Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits. Exp Ther Med. 2016,12(1):51-58.
    Read this paper
  • Wang JQ, Li XW, Liu M, et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma. Mol Med Rep. 2016,13(5):4289-302. doi: 10.3892/mmr.2016.5050
    Read this paper
  • Tang LQ, Ni WJ, Cai M, et al. Renoprotective effects of berberine and its potential effect on the expression of β-arrestins and intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in streptozocin-diabetic nephropathy rats. J Diabetes. 2016,8(5):693-700. doi: 10.1111/1753-0407.12349
    Read this paper
  • Wang X, Shi H, Cui J, et al. Preliminary Research of Relationship between Acute Peritonitis and Celiac Primo Vessels. Evid Based Complement Alternat Med. 2013,2013:569161. doi: 10.1155/2013/569161
    Read this paper
  • Zhang XM, Lv YG, Chen GB, et al. Effect of mild hypothermia on breast cancer cells adhesion and migration. Biosci Trends. 2012,6(6):313-24.
    Read this paper

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Figure 1. Western blot analysis of Icam1 using anti-Icam1 antibody (A00171-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Icam1 antigen affinity purified polyclonal antibody (Catalog # A00171-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Icam1 at approximately 115KD. The expected band size for Icam1 is at 115KD.
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