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ProductsBack/GenieFluor 488 Anti-Mouse CD54 Antibody [YN1/1.7.4]

GenieFluor 488 Anti-Mouse CD54 Antibody [YN1/1.7.4]

AGEL0121
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
TargetIcam1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Mouse CD54 Monoclonal Antibody (Alexa Fluor 488 Conjugated) [YN1/1.7.4] (AGEL0121)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    AlexaFluor 488
  • Gene ID15894
  • Target name
    Icam1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    CD54, Icam-1, Ly-47, MALA-2, intercellular adhesion molecule 1, myD10
  • Host
    Rat
  • Protein IDP13597
  • Protein Name
    Intercellular adhesion molecule 1
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Icam1 using anti-Icam1 antibody (A00171-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Icam1 antigen affinity purified polyclonal antibody (Catalog # A00171-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Icam1 at approximately 115KD. The expected band size for Icam1 is at 115KD.
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