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ProductsBack/GenieFluor 488 Anti-Mouse CD54 Antibody [YN1/1.7.4]

GenieFluor 488 Anti-Mouse CD54 Antibody [YN1/1.7.4]

AGEL0121
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetIcam1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    GenieFluor 488 Anti-Mouse CD54 Antibody [YN1/1.7.4]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    YN1/1.7.4
  • Conjugate
    Other Conjugate
  • Gene ID15894
  • Target name
    Icam1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    CD54, Icam-1, Ly-47, MALA-2, intercellular adhesion molecule 1, myD10
  • Host
    Rat
  • Protein IDP13597
  • Protein Name
    Intercellular adhesion molecule 1
  • Scientific Description
    GenieFluor 488 Anti-Mouse CD54 Antibody [YN1/1.7.4] is a high-performance monoclonal that specifically targets Mouse CD54, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Available with AF488 conjugation for enhanced detection sensitivity and experimental flexibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Icam1 using anti-Icam1 antibody (A00171-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Icam1 antigen affinity purified polyclonal antibody (Catalog # A00171-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Icam1 at approximately 115KD. The expected band size for Icam1 is at 115KD.
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