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Western blot All lanes: ICAM5 antibody at 4.2ug/ml Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa
Western blot All lanes: ICAM5 antibody at 4.2ug/ml Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa
Western blot All lanes: ICAM5 antibody at 4.2ug/ml Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa

ICAM5 Antibody

CSB-PA892475ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetICAM5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ICAM5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7087
  • Target name
    ICAM5
  • Target description
    intercellular adhesion molecule 5
  • Target synonyms
    TLCN, TLN, intercellular adhesion molecule 5, ICAM-5, intercellular adhesion molecule 5, telencephalin, telencephalin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMF0
  • Protein Name
    Intercellular adhesion molecule 5
  • Scientific Description
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ICAM5 using anti-ICAM5 antibody (A07187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM5 antigen affinity purified polyclonal antibody (Catalog # A07187-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM5 at approximately 140 kDa. The expected band size for ICAM5 is at 97 kDa.
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