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ProductsBack/IDE Rabbit Polyclonal Antibody
Western blot analysis of IDE in HepG2 lysates using Insulin Degrading Enzyme antibody.
Western blot analysis of IDE in HepG2 lysates using Insulin Degrading Enzyme antibody.
Western blot analysis of IDE in HepG2 lysates using Insulin Degrading Enzyme antibody.

IDE Rabbit Polyclonal Antibody

TA384478
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIDE
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    IDE Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3416
  • Target name
    IDE
  • Target description
    insulin degrading enzyme
  • Target synonyms
    INSULYSIN, insulin-degrading enzyme, Abeta-degrading protease, insulin protease, insulinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14735
  • Protein Name
    Insulin-degrading enzyme
  • Scientific Description
    Insulin Degrading Enzyme Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
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