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ProductsBack/IFIX / PYHIN1 Antibody (HRP)

IFIX / PYHIN1 Antibody (HRP)

LS-C399126
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetPYHIN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IFIX / PYHIN1 Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID149628
  • Target name
    PYHIN1
  • Target description
    pyrin and HIN domain family member 1
  • Target synonyms
    IFIX; interferon-inducible protein X; pyrin and HIN domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYHIN1 using anti-PYHIN1 antibody (A10377-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYHIN1 antigen affinity purified polyclonal antibody (Catalog # A10377-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYHIN1 at approximately 55 kDa. The expected band size for PYHIN1 is at 55 kDa.
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