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PYHIN1 Antibody

PACO36818
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPYHIN1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PYHIN1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID149628
  • Target name
    PYHIN1
  • Target description
    pyrin and HIN domain family member 1
  • Target synonyms
    IFIX, pyrin and HIN domain-containing protein 1, interferon-inducible protein X
  • Host
    Rabbit
  • Protein IDQ6K0P9
  • Protein Name
    Pyrin and HIN domain-containing protein 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of PYHIN1 using anti-PYHIN1 antibody (A10377-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYHIN1 antigen affinity purified polyclonal antibody (Catalog # A10377-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYHIN1 at approximately 55 kDa. The expected band size for PYHIN1 is at 55 kDa.
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