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IGF2BP3 antibody

10R-10952
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    IGF2BP3 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HEK293 whole cell lysate, Mouse brain tissue All lanes: IGF2BP3 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64, 22 kDa Observed band size: 64, 50 kDa
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Figure 1. Western blot analysis of IMP3/IGF2BP3 using anti-IMP3/IGF2BP3 antibody (A02362-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP3/IGF2BP3 antigen affinity purified polyclonal antibody (Catalog # A02362-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP3/IGF2BP3 at approximately 64 kDa. The expected band size for IMP3/IGF2BP3 is at 64 kDa.
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