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ProductsBack/IMP3 Recombinant Antibody, AbBy Fluor-594 Conjugated

IMP3 Recombinant Antibody, AbBy Fluor-594 Conjugated

BSM-61766R-BF594
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetIGF2BP3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IMP3 Recombinant Antibody, AbBy Fluor-594 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID10643
  • Target name
    IGF2BP3
  • Target description
    insulin like growth factor 2 mRNA binding protein 3
  • Target synonyms
    cancer/testis antigen 98; CT98; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; IMP3; IMP-3; insulin-like growth factor 2 mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KOC; KOC1; VICKZ family member 3; VICKZ3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00425
  • Protein Name
    Insulin-like growth factor 2 mRNA-binding protein 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IMP3/IGF2BP3 using anti-IMP3/IGF2BP3 antibody (A02362-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP3/IGF2BP3 antigen affinity purified polyclonal antibody (Catalog # A02362-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP3/IGF2BP3 at approximately 64 kDa. The expected band size for IMP3/IGF2BP3 is at 64 kDa.
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