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Western Blot analysis of MCF7 cells using IGFBP1 Polyclonal Antibody.
Western Blot analysis of MCF7 cells using IGFBP1 Polyclonal Antibody.
Western Blot analysis of MCF7 cells using IGFBP1 Polyclonal Antibody.

IGFBP1 Antibody

CSB-PA006134
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIGFBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IGFBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3484
  • Target name
    IGFBP1
  • Target description
    insulin like growth factor binding protein 1
  • Target synonyms
    AFBP; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; IGF-BP25; insulin-like growth factor-binding protein 1; placental protein 12; PP12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08833
  • Protein Name
    Insulin-like growth factor-binding protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (A00922). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody (Catalog # A00922) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30 kDa. The expected band size for IGFBP1 is at 28 kDa.
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