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Western Blot analysis of MOUSE-BRAIN cells using IGFBP3 Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN cells using IGFBP3 Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN cells using IGFBP3 Polyclonal Antibody

IGFBP3 Antibody

CSB-PA003004
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetIGFBP3
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Overview

  • Supplier
    Cusabio
  • Product Name
    IGFBP3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3486
  • Target name
    IGFBP3
  • Target description
    insulin like growth factor binding protein 3
  • Target synonyms
    BP-53, IBP-3, IBP3, IGFBP-3, insulin-like growth factor-binding protein 3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex, binding protein 29, binding protein 53, growth hormone-dependent binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17936
  • Protein Name
    Insulin-like growth factor-binding protein 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IGFBP3 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.
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