![IGFBP3 antibody [N2C3] detects secreted IGFBP3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IGFBP3 stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min IGFBP3 antibody [N2C3] detects secreted IGFBP3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IGFBP3 stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100454/GTX100454_44643_20220624_IHC-P_R_22062919_240.webp)
IGFBP3 antibody [N2C3] detects secreted IGFBP3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IGFBP3 stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
IGFBP3 antibody [N2C3]
GTX100454
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIGFBP3
Overview
- SupplierGeneTex
- Product NameIGFBP3 antibody [N2C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.31 mg/ml
- ConjugateUnconjugated
- Gene ID3486
- Target nameIGFBP3
- Target descriptioninsulin like growth factor binding protein 3
- Target synonymsBP-53, IBP-3, IBP3, IGFBP-3, insulin-like growth factor-binding protein 3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex, binding protein 29, binding protein 53, growth hormone-dependent binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP17936
- Protein NameInsulin-like growth factor-binding protein 3
- Scientific DescriptionThis gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Shih HJ, Chang HF, Chen CL, et al. Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer. Sci Rep. 2021,11(1):22925. doi: 10.1038/s41598-021-02400-1Read this paper
- Shih HJ, Chen CL, Torng PL. IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Am J Cancer Res. 2020,10(6):1728-1744.Read this paper
- Johnson TK, Zhao L, Zhu D, et al. Exosomes derived from induced vascular progenitor cells promote angiogenesis in vitro and in an in vivo rat hindlimb ischemia model. Am J Physiol Heart Circ Physiol. 2019,317(4):H765-H776. doi: 10.1152/ajpheart.00247.2019Read this paper
- Wang HH, Wang YC, Wu DW, et al. Targeting insulin-like growth factor-binding protein-3 by microRNA-125b promotes tumor invasion and poor outcomes in non-small-cell lung cancer. Tumour Biol. 2017,39(4):1010428317694316. doi: 10.1177/1010428317694316Read this paper
![IGFBP3 antibody [N2C3] detects IGFBP3 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: IGFBP3 protein stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). IGFBP3 antibody [N2C3] detects IGFBP3 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: IGFBP3 protein stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX100454/GTX100454_40604_20160921_IHC-Fr_w_23060100_135.webp)
![U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100454/GTX100454_40604_20211119_WB_Fraction_w_23060100_522.webp)



![U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody [HL1505] (GTX636979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636979/GTX636979_T-44676_20220513_WB_Fraction_w_23061202_477.webp)



