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Western Blot analysis of K562 cells using CD179b Polyclonal Antibody
Western Blot analysis of K562 cells using CD179b Polyclonal Antibody
Western Blot analysis of K562 cells using CD179b Polyclonal Antibody

IGLL1/IGLC1/IGLC2/IGLC3/IGLC6/IGLC7 Antibody

CSB-PA001429
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIGLL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IGLL1/IGLC1/IGLC2/IGLC3/IGLC6/IGLC7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3543
  • Target name
    IGLL1
  • Target description
    immunoglobulin lambda like polypeptide 1
  • Target synonyms
    14.1, AGM2, CD179b, IGL1, IGL5, IGLJ14.1, IGLL, IGO, IGVPB, VPREB2, immunoglobulin lambda-like polypeptide 1, CD179 antigen-like family member B, CD179b antigen, Pre-B lymphocyte-specific protein-2, ig lambda-5, immunoglobulin omega polypeptide chain, immunoglobulin-related 14.1 protein, immunoglobulin-related protein 14.1, lambda5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA0M8Q6
  • Protein Name
    Immunoglobulin lambda constant 7
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IGLL1 using anti-IGLL1 antibody (A08965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGLL1 antigen affinity purified polyclonal antibody (Catalog # A08965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGLL1 at approximately 26KD. The expected band size for IGLL1 is at 23KD.
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Immunohistochemistry analysis in human bone marrow and cerebral cortex tissues using Anti-IGLL1 antibody. Corresponding IGLL1 RNA-seq data are presented for the same tissues.
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