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ProductsBack/IGLL1/IGLC1/IGLC2/IGLC3/IGLC6/IGLC7 Antibody

IGLL1/IGLC1/IGLC2/IGLC3/IGLC6/IGLC7 Antibody

PACO00550
Assay Genie
ApplicationsWestern Blot, ELISA
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIGLL1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    IGLL1/IGLC1/IGLC2/IGLC3/IGLC6/IGLC7 Antibody (PACO00550)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3543
  • Target name
    IGLL1
  • Target description
    immunoglobulin lambda like polypeptide 1
  • Target synonyms
    14.1; AGM2; CD179 antigen-like family member B; CD179b; CD179b antigen; ig lambda-5; IGL1; IGL5; IGLJ14.1; IGLL; IGO; IGVPB; immunoglobulin lambda-like polypeptide 1; immunoglobulin omega polypeptide chain; immunoglobulin-related 14.1 protein; immunoglobulin-related protein 14.1; lambda5; Pre-B lymphocyte-specific protein-2; VPREB2
  • Host
    Rabbit
  • Protein IDA0M8Q6
  • Protein Name
    Immunoglobulin lambda constant 7
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Mouse Stomach lysates; Lane 2: Mouse Large intestine lysates probed with IGLL1 Polyclonal Antibody, Unconjugated (bs-15577R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C._x000D_
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Figure 1. Western blot analysis of IGLL1 using anti-IGLL1 antibody (A08965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGLL1 antigen affinity purified polyclonal antibody (Catalog # A08965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGLL1 at approximately 26KD. The expected band size for IGLL1 is at 23KD.
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