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ProductsBack/IHC-plus(tm) ABCB1 / MDR1 / P Glycoprotein Antibody

IHC-plus(tm) ABCB1 / MDR1 / P Glycoprotein Antibody

Research Use Only
LS-B1448
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetABCB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ABCB1 / MDR1 / P Glycoprotein Antibody
  • Delivery Days Customer
    23
  • Antibody Specificity
    A BLAST analysis was used to suggest cross reactivity with ABCB1 protein from human, monkey, dog, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
  • Application Supplier Note
    Tested in Western blot with recombinant proteins only. Immunohistochemistry: LS-B1448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1448 was determined to be 5 ug/ml.. ELISA (1:10000 - 1:50000), IHC, IHC-P (5 µg/ml), WB (1:200 - 1:2000) Tested in Western blot with recombinant proteins only. Immunohistochemistry: LS-B1448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1448 was determined to be 5 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5243
  • Target name
    ABCB1
  • Target description
    ATP binding cassette subfamily B member 1
  • Target synonyms
    ABC20; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; GP170; MDR1; multidrug resistance protein 1; p-170; P-glycoprotein 1; P-GP; PGY1; phospholipid transporter ABCB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • UNSPSC
    12352203