IHC-plus(tm) ABCB1 / MDR1 / P Glycoprotein Antibody

LifeSpan BioSciences

IHC-plus(tm) ABCB1 / MDR1 / P Glycoprotein Antibody

23

Tested in Western blot with recombinant proteins only. Immunohistochemistry: LS-B1448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1448 was determined to be 5 ug/ml.. ELISA (1:10000 - 1:50000), IHC, IHC-P (5 µg/ml), WB (1:200 - 1:2000) Tested in Western blot with recombinant proteins only. Immunohistochemistry: LS-B1448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1448 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

ABCB1

ATP binding cassette subfamily B member 1

ABC20; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; GP170; MDR1; multidrug resistance protein 1; p-170; P-glycoprotein 1; P-GP; PGY1; phospholipid transporter ABCB1

Rabbit

IgG

12352203