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IHC-plus(tm) ADAM19 Antibody

LS-B9389
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetADAM19
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ADAM19 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:250)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8728
  • Target name
    ADAM19
  • Target description
    ADAM metallopeptidase domain 19
  • Target synonyms
    FKSG34, MADDAM, MLTNB, disintegrin and metalloproteinase domain-containing protein 19, a disintegrin and metalloproteinase domain 19 (meltrin beta), metalloprotease and disintegrin dendritic antigen marker, metalloprotease-disintegrin meltrin beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ADAM19 using anti-ADAM19 antibody (PA2070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM19 antigen affinity purified polyclonal antibody (Catalog # PA2070) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM19 at approximately 105 kDa. The expected band size for ADAM19 is at 105 kDa.
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