IHC-plus(tm) ALOX5 / 5-LOX Antibody (aa130-149)

LifeSpan BioSciences

IHC-plus(tm) ALOX5 / 5-LOX Antibody (aa130-149)

23

Immunohistochemistry: LS-B1499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1499 was determined to be 10 ug/ml.. ICC, IHC, IHC-P (1:50 - 1:100), WB Immunohistochemistry: LS-B1499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1499 was determined to be 10 ug/ml.

Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

ALOX5

arachidonate 5-lipoxygenase

5-LO, 5-LOX, 5LPG, LOG5, polyunsaturated fatty acid 5-lipoxygenase, LOX-5, arachidonic acid 5-lipoxygenase, leukotriene A4 synthase

Rabbit

IgG

Bovine, Human, Rat

-20°C,2°C to 8°C

41116161