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IHC-plus(tm) AMBP Antibody

LS-B13863
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAMBP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) AMBP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 38kDa, while the observed MW by Western blot was 46kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 38kDa, while the observed MW by Western blot was 46kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID259
  • Target name
    AMBP
  • Target description
    alpha-1-microglobulin/bikunin precursor
  • Target synonyms
    A1M; bikunin; complex-forming glycoprotein heterogeneous in charge; EDC1; growth-inhibiting protein 19; HCP; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate All lanes: AMBP antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
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Figure 1. Western blot analysis of Bikunin/AMBP using anti-Bikunin/AMBP antibody (A02419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bikunin/AMBP antigen affinity purified polyclonal antibody (Catalog # A02419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bikunin/AMBP at approximately 34KD. The expected band size for Bikunin/AMBP is at 39KD.
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