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IHC-plus(tm) ATP5H Antibody (aa111-160)

LS-B9712
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP5PD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ATP5H Antibody (aa111-160)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 18 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), Peptide-ELISA (1:20000), WB (1:500 - 1:1000) Western Blot: Predicted MW 18 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10476
  • Target name
    ATP5PD
  • Target description
    ATP synthase peripheral stalk subunit d
  • Target synonyms
    APT5H, ATP5H, ATPQ, ATP synthase peripheral stalk subunit d, mitochondrial, ATP synthase D chain, mitochondrial, ATP synthase subunit d, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, ATP synthase, H+ transporting, mitochondrial F1F0, subunit d, ATP synthase, H+ transporting, mitochondrial Fo complex subunit D, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d, ATPase subunit d, My032 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of ATP5H using anti-ATP5H antibody (PB9328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5H antigen affinity purified polyclonal antibody (Catalog # PB9328) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22 kDa. The expected band size for ATP5H is at 22 kDa.
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