IHC-plus(tm) BAFF / TNFSF13B Antibody

LifeSpan BioSciences

IHC-plus(tm) BAFF / TNFSF13B Antibody

23

Immunohistochemistry: LS-B8201 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 5-6 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with biotinylated antigen affinity purified anti-human BAFF (LS-C104386) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2-4 ng/lane, under non-reducing conditions.. ELISA (5 - 6 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.25 - 0.5 µg/ml) Immunohistochemistry: LS-B8201 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 5-6 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with biotinylated antigen affinity purified anti-human BAFF (LS-C104386) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2-4 ng/lane, under non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

TNFSF13B

TNF superfamily member 13b

BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4, tumor necrosis factor ligand superfamily member 13B, ApoL related ligand TALL-1, B-cell-activating factor, B-lymphocyte stimulator, Delta4 BAFF, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, delta BAFF, dendritic cell-derived TNF-like molecule, epididymis secretory sperm binding protein, tumor necrosis factor (ligand) superfamily, member 13b, tumor necrosis factor (ligand) superfamily, member 20, tumor necrosis factor ligand 7A, tumor necrosis factor superfamily member 13b, tumor necrosis factor-like protein ZTNF4

Mouse

IgG1

Human

-20°C,2°C to 8°C,RT

41116161