IHC-plus(tm) BIRC5 / Survivin Antibody (clone 60.11)

LifeSpan BioSciences

IHC-plus(tm) BIRC5 / Survivin Antibody (clone 60.11)

23

Immunohistochemistry: LS-B499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B499 was determined to be 2.5-5 ug/ml.. IHC, IHC-P (2.5 - 5 µg/ml), WB (1:1000 - 1:3000) Immunohistochemistry: LS-B499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B499 was determined to be 2.5-5 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

60.11

0.9 mg/ml

Unconjugated

...

BIRC5

baculoviral IAP repeat containing 5

API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin

Mouse

IgG2a

-20°C or -80°C

12352203