IHC-plus(tm) BRCA1 Antibody (Ser1423)
LS-B1176
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBRCA1
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) BRCA1 Antibody (Ser1423)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B1176 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1176 was determined to be 20 ug/ml.. IHC, IHC-P (20 µg/ml), WB Immunohistochemistry: LS-B1176 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1176 was determined to be 20 ug/ml.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID672
- Target nameBRCA1
- Target descriptionBRCA1 DNA repair associated
- Target synonymsBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53, breast cancer type 1 susceptibility protein, BRCA1/BRCA2-containing complex, subunit 1, Fanconi anemia, complementation group S, RING finger protein 53, breast and ovarian cancer susceptibility protein 1, breast cancer 1, early onset, early onset breast cancer 1, protein phosphatase 1, regulatory subunit 53
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C,-80°C
- UNSPSC41116161

![A549 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [C3], C-term (GTX100720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100720/GTX100720_39988_20180504_WB_Fraction_w_23060100_697.webp)



