IHC-plus(tm) BTC / Betacellulin Antibody

LifeSpan BioSciences

IHC-plus(tm) BTC / Betacellulin Antibody

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Immunohistochemistry: LS-B7481 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Betacellulin (0.1 ng/ml), a concentration of &le. 0.1 ug/ml of this antibody is required. ELISA: To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Betacellulin (LS-C104290) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBetacellulin. Western Blot: To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7481 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Betacellulin (0.1 ng/ml), a concentration of &le. 0.1 ug/ml of this antibody is required. ELISA: To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Betacellulin (LS-C104290) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBetacellulin. Western Blot: To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

BTC

betacellulin

probetacellulin

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

41116161