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IHC-plus(tm) CAPN3 / Calpain 3 Antibody

LS-B14508
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCAPN3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CAPN3 / Calpain 3 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID825
  • Target name
    CAPN3
  • Target description
    calpain 3
  • Target synonyms
    CANP3, CANPL3, LGMD2, LGMD2A, LGMDD4, LGMDR1, nCL-1, p94, calpain-3, calpain p94, large [catalytic] subunit, calpain, large polypeptide L3, muscle-specific calcium-activated neutral protease 3 large subunit, new calpain 1
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Calpain 3/CAPN3 using anti-Calpain 3/CAPN3 antibody (A02170-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 3/CAPN3 antigen affinity purified polyclonal antibody (Catalog # A02170-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 3/CAPN3 at approximately 99 kDa. The expected band size for Calpain 3/CAPN3 is at 99 kDa.
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