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IHC-plus(tm) CASP8 / Caspase 8 Antibody

LS-B13015
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCASP8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CASP8 / Caspase 8 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID841
  • Target name
    CASP8
  • Target description
    caspase 8
  • Target synonyms
    ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5, caspase-8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein, MORT1-associated ced-3 homolog, apoptotic cysteine protease, apoptotic protease Mch-5, caspase 8, apoptosis-related cysteine peptidase, caspase 8, apoptosis-related cysteine protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.
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Anti-Caspase 8/CASP8 Antibody Picoband(r)A00042-1-CARRIER-FREE
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TargetCASP8
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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ReactivityCanine, Feline, Human, Mouse, Rat
TargetCASP8
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TargetCASP8
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References
Bioss
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TargetCASP8
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