IHC-plus(tm) CCR6 Antibody (aa18-46)
LS-B2481
ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCCR6
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) CCR6 Antibody (aa18-46)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B2481 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2481 was determined to be 5 ug/ml. ELISA: 1:75000. Flow Cytometry: 50-100ul, 1-3 ul per 10^6 cells.. ELISA (1:75000), Flo (1 - 3 µg/10E6 cells), ICC (1:300), IHC, IHC-P (5 µg/ml) Immunohistochemistry: LS-B2481 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2481 was determined to be 5 ug/ml. ELISA: 1:75000. Flow Cytometry: 50-100ul, 1-3 ul per 10^6 cells.
- ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1235
- Target nameCCR6
- Target descriptionC-C motif chemokine receptor 6
- Target synonymsBN-1, C-C CKR-6, CC-CKR-6, CCR-6, CD196, CKR-L3, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22, C-C chemokine receptor type 6, G protein-coupled receptor 29, LARC receptor, chemokine (C-C motif) receptor 6, chemokine (C-C) receptor 6, chemokine receptor-like 3, seven-transmembrane receptor, lymphocyte, 22
- HostGoat
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC41116161





![Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCR6 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-AF594) at [1:500] for 90 minutes and DAPI staining of the nuclei.](https://biossantibodies.com/image-raw/12822.jpeg)

