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ProductsBack/IHC-plus(tm) CD45 / LCA Antibody

IHC-plus(tm) CD45 / LCA Antibody

LS-B15351
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPTPRC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CD45 / LCA Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 130kDa/147kDa, while the observed MW by Western blot was 185kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:400), WB (1:500 - 1:2000) The predicted MW is 130kDa/147kDa, while the observed MW by Western blot was 185kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.57 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5788
  • Target name
    PTPRC
  • Target description
    protein tyrosine phosphatase receptor type C
  • Target synonyms
    B220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200, receptor-type tyrosine-protein phosphatase C, CD45 antigen, T200 glycoprotein, T200 leukocyte common antigen, glycoprotein 180, protein tyrosine phosphatase, receptor type, c polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (A00555-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45/PTPRC antigen affinity purified polyclonal antibody (Catalog # A00555-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45/PTPRC at approximately 220 kDa. The expected band size for CD45/PTPRC is at 220 kDa.
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