IHC-plus(tm) CDC25A Antibody (aa271-300)

LifeSpan BioSciences

IHC-plus(tm) CDC25A Antibody (aa271-300)

23

Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml), WB (1:1000) Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

CDC25A

cell division cycle 25A

CDC25A2, M-phase inducer phosphatase 1, CDC25 homolog A, dual specificity phosphatase CDC25A

Rabbit

Human

-20°C,2°C to 8°C

41116161