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ProductsBack/IHC-plus(tm) CDCP1 Antibody (aa637-649)

IHC-plus(tm) CDCP1 Antibody (aa637-649)

LS-B15178
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCDCP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CDCP1 Antibody (aa637-649)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 90 kDa band observed in Human Colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunising peptide.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 90 kDa band observed in Human Colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunising peptide.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64866
  • Target name
    CDCP1
  • Target description
    CUB domain containing protein 1
  • Target synonyms
    CD318, SIMA135, TRASK, CUB domain-containing protein 1, membrane glycoprotein gp140, subtractive immunization M plus HEp3-associated 135 kDa protein, transmembrane and associated with src kinases
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDCP1 using anti-CDCP1 antibody (A02411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCP1 antigen affinity purified polyclonal antibody (Catalog # A02411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCP1 at approximately 130 kDa. The expected band size for CDCP1 is at 93 kDa.
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