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ProductsBack/IHC-plus(tm) CFD / Factor D / Adipsin Antibody

IHC-plus(tm) CFD / Factor D / Adipsin Antibody

LS-B15775
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCFD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CFD / Factor D / Adipsin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 27kDa, while the observed MW by Western blot was 27kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 27kDa, while the observed MW by Western blot was 27kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.954 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1675
  • Target name
    CFD
  • Target description
    complement factor D
  • Target synonyms
    ADIPSIN, ADN, DF, PFD, complement factor D, C3 convertase activator, D component of complement (adipsin), complement factor D preproprotein, properdin factor D
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CFD using anti-CFD antibody (A01134-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFD antigen affinity purified polyclonal antibody (Catalog # A01134-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFD at approximately 45 kDa. The expected band size for CFD is at 27 kDa.
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