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ProductsBack/IHC-plus(tm) CHD4 Antibody (clone 4H4)

IHC-plus(tm) CHD4 Antibody (clone 4H4)

LS-B4300
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCHD4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CHD4 Antibody (clone 4H4)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control HeLa NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), WB Western Blot (Cell lysate) - positive control HeLa NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4H4
  • Conjugate
    Unconjugated
  • Gene ID1108
  • Target name
    CHD4
  • Target description
    chromodomain helicase DNA binding protein 4
  • Target synonyms
    CHD-4, Mi-2b, Mi2-BETA, SIHIWES, chromodomain-helicase-DNA-binding protein 4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHD4 using anti-CHD4 antibody (A01125-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD4 antigen affinity purified polyclonal antibody (Catalog # A01125-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD4 at approximately 260 kDa. The expected band size for CHD4 is at 218 kDa.
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