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ProductsBack/IHC-plus(tm) CHRNA5 Antibody (aa369-380)

IHC-plus(tm) CHRNA5 Antibody (aa369-380)

LS-B8364
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetCHRNA5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CHRNA5 Antibody (aa369-380)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kD band observed in lysates of cell line KELLY (calculated MW of 53.1kD according to NP_000736.2). Recommended concentration: 1-3 ug/ml.. IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kD band observed in lysates of cell line KELLY (calculated MW of 53.1kD according to NP_000736.2). Recommended concentration: 1-3 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1138
  • Target name
    CHRNA5
  • Target description
    cholinergic receptor nicotinic alpha 5 subunit
  • Target synonyms
    LNCR2, neuronal acetylcholine receptor subunit alpha-5, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, acetylcholine receptor, nicotinic, alpha 5 (neuronal), cholinergic receptor, nicotinic alpha 5, cholinergic receptor, nicotinic, alpha 5 (neuronal), neuronal nicotinic acetylcholine receptor, alpha5 subunit
  • Host
    Goat
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat skeletal muscle tissue lysates, lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA5 antigen affinity purified polyclonal antibody (Catalog # A02359-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRNA5 at approximately 53KD. The expected band size for CHRNA5 is at 53KD.
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